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. 2019 Dec 17;7(1):121–125. doi: 10.1002/acn3.50949

Figure 2.

Figure 2

Mouse in vivo study: Mcf2 knock‐down (KD) alters the laminar positioning of projection neurons (PNs) at P0.5 and the G4A missense mutation alters the migratory function of MCF2. (A) The overexpression of hMCF2 by E14.5 electroporation in S1 PNs colabeled with Tomato (TOM) displays a cytoplasmic expression at P0.5. (B) q‐RT‐PCR of mRNA extracts from HEK‐293T cells cotransfected with mMcf2 and Mcf2 or Scramble (Scram) shRNA shows a 40% knock‐down (KD) of the mRNA expression by Mcf2 shRNA, compared to Scram shRNA. Error bars = 95% C.I. (C) shRNA‐mediated Mcf2 KD by in utero electroporation at E14.5 dramatically impairs the laminar positioning of E14.5‐electroporated PNs coexpressing TOM in S1 at P0.5. This phenotype is fully rescued by the overexpression of the shRNA‐resistant hMCF2 but the congenital bilateral perisylvian syndrome (CBPS)‐associated missense mutation G4A prevents any rescue. n = 6–11 brains per condition from ≥3 separate litters. Error bars = 95% CI.