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. 2019 Dec 14;98(1):135–148. doi: 10.1007/s00109-019-01859-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — B7H6 induction by ER stress is correlated with its mRNA levels. a Schematic representation of the 5′UTR (untranslated region) of B7H6 (5′BG, upper panel), the lower panel shows the mutated nucleotide from U to A, AAG5′BG. b Measurement of GFP levels by flow cytometry in 624 wt cells stably expressing empty vector, 5′BG or AAG5′BG constructs after treatment with 0.125 μg/ml Tg or mock treated with DMSO for 16 h. c Polysome profiling of B7H6 mRNA in 624 wt or PERK KO cells after treatment with 0.125 μg/ml Tg or mock treated with DMSO for 16 h, presented is the average ± STD of triplicates of the mRNA levels of B7H6 (middle panel) and ATF4 (lower panel) in the different sucrose fractions. d Real-time PCR quantification of B7H6 mRNA in 624 wt or PERK KO cells after treatment with 0.125 μg/ml Tg or mock treated with DMSO for 16 h, represented is the average of relative normalized mRNA levels ± STD of three independent experiments. The right panel represents B7H6 surface levels on the tested cells (BG indicates secondary only background staining, shown is the BG for untreated cells)