Fig. 5.

Treatment with Tg or Nelfinavir enhanced the activity of B7H6-directed CAR-T cells. a IFNγ concentration (pg/ml) secreted by B7H6 specific CAR T or control T cells after 24 h of co-culture with 624 wt cells pretreated with 0.125 μg/ml Tg, 10 μM Nel, or 20 μM Lop for 16 h. CAR T cells treated with 20 ng/ml PMA and μg/ml ionomycin were used as positive control and CAR T cells that were not cultured with 624 cells were used as negative control (IFNγ levels for the positive and the negative controls are not shown). b % specific cytotoxicity for the target cells in a calculated by LDH activity as follows: %cytotoxicity = [(treated LDH activity) − (spontaneous LDH activity)]/[(maximum LDH activity) − (spontaneous LDH activity)] * 100. The bars represent the average ± STD of triplicates of CAR T cells from three different donors. c IFNγ concentration (pg/ml) secreted by B7H6-specific CAR T or control T cells after 24 h of co-culture with 624 wt or B7H6 KO cells under control or Tg treatment as in a. d B7H6-specific contribution to the CAR T cell activity. This contribution was calculated by subtracting the IFNγ concentration values of the KO cells from the concentration values of wt cells for DMSO or Tg-treated cells. Statistical significance was determined by Kruskal–Wallis one-way analysis of variance at *p < 0.05