Figure 6.

Exosome-mediated effects on NKG2D receptor expression levels on human primary NK cells. In (a), total exosomes, MTEX or non-MTEX were co-incubated with human activated NK cells for 24 h. Representative data of exosome-mediated suppression of NKG2D levels by exosomes from one melanoma patient are shown. In (b), % of NK cells expressing NKG2D after co-incubation with exosomes of HDs or total exosomes, MTEX or non-MTEX from melanoma patients’ plasma. In (c), the presence of MICA or MICB (determined as RFI) on MTEX and non-MTEX. All data were normalized to no exosome (PBS) controls. Blocking of MTEX-mediated downregulation of NKG2D on NK cells by neutralizing anti-TGF-β Ab is shown in SFig. 8. Horizontal bars indicate median values. NS: no significant difference. Wilcoxon signed-rank tests were used to evaluate differences between paired samples; Wilcoxon-Mann-Whitney tests were used to evaluate differences between patients and healthy donors.