Skip to main content
. 2019 Nov 27;17(1):110. doi: 10.1038/s41423-019-0336-8

Fig. 5.

Fig. 5

LPS activates Stat3 in an IL-6 independent manner. a Primary macrophages from IL-6−/− and WT mice were treated with LPS (100 ng/ml) for the indicated time points. p-Stat3 and Stat3 were analyzed using immunoblotting. b Primary macrophages were treated with IL-6 antibody or mouse normal IgG and subsequently treated with LPS for the indicated time points. p-Stat3 and Stat3 were analyzed through immunoblotting. c–e Primary macrophages were treated with CHX, ACTD or BFA for eight hours and then stimulated with LPS (100 ng/ml) for the indicated time points. p-Stat3 and Stat3 levels were analyzed using immunoblotting.