Fig. 2.

HSP70L1 and DNAJC2 interact with and stabilize each other. a Coimmunoprecipitation analysis of the interaction between HSP70L1 and DNAJC2 at indicated time during the differentiation of MoDCs. b, c Real-time PCR (b) and WB (c) analyses of the expression of HSP70L1 and DNAJC2 in MoDCs transfected with siRNAs of control (siNC), HSP70L1 (siHSP70L1) or DNAJC2 (siDNAJC2) on day 4 for 48 h (PCR) or 72 h (WB), respectively. Data are normalized to actin (b), GAPDH (c, cytoplasmic lysate), or LaminA/C (c, nuclear lysate), and siNC group is set as 1 (c). d, e Real-time PCR (d) and WB (e) analyses of the expression of DNAJC2 at indicated time during the differentiation of MoDCs. Data are normalized to GAPDH (d, e, whole cell and cytoplasmic lysate) or Histone H3 (e, nuclear lysate), and day 0 group is set as 1. f Confocal microscopy analysis of the expression of HSP70L1 and DNAJC2 in MoDCs on days 3 and 6, respectively (TCS-SP8, Leica, Wetzlar, Germany). Bars are indicated in figures. Data are shown as the mean ±s.e.m. of three experiments (b, d). One representative result of two (a, f) or three (c, e) independent experiments is shown. ***P < 0.001 compared to siNC group (t test)