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. 2020 Jan 9;11:167. doi: 10.1038/s41467-019-13905-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Experimental setup for the single shot diffractive imaging measurements of sucrose clusters. The clusters intersect the focused FEL pulse and their coherent diffraction image is recorded with a large area pixel detector. Upstream of the focusing optics the energy of each XFEL pulse is measured and recorded with the scattering data. The clusters are small compared to the focus diameter and their location within the focal volume determines the incident fluence on them.