Fig. 2. Simulation and estimation of sample coverage in a multi-view dataset.

a The simulated sample coverage with a single illumination and a single detection objective. b The simulated sample coverage with four views surrounding the sample. c The two methods to perform multi-view imaging: Multiple objectives surrounding the sample or multiple views created by rotating the sample. Illustration of variable definitions used in Eq. (1). d Estimated angular fluorophore distribution of a 2 day old zebrafish embryo. e Estimated optical accessibility of a 2 day old zebrafish embryo. f Estimated sample coverage of a real zebrafish embryo measured by number of foreground blocks.