Fig. 4.

Ex vivo DC-10 secrete IL-10 spontaneously and upon activation. Ex vivo DC-10 (CD14⁺CD16⁺CD141⁺CD163⁺), classical (CD14⁺CD16⁻) and non-classical (CD14⁺CD16⁺CD163⁻) monocytes were FACS-isolated from the peripheral blood of healthy donors and left unstimulated or stimulated with LPS for 48 h (n = 9). Concentration levels of IL-10, IL-12, TNF-α, and IL-6 in culture supernatants were evaluated by a multiplex microbead-based cytokine array. Each dot represents a single donor, lines indicate medians, and whiskers are minimum and maximum levels. *P ≤ 0.05, **P ≤ 0.01 (Wilcoxon matched pairs test, two-tailed)