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. 2020 Jan 9;10:83. doi: 10.1038/s41598-019-57053-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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S-SCAM overexpression causes the loss of GABAergic synapses. (a) Representative immunofluorescent images of GABA_AR γ2 and vGAT in the dendrites of hippocampal neurons transfected with plasmids expressing either control (mCherry) or myc-S-SCAM. (b−d) Quantification of densities (b) and intensities (c) of GABA_AR γ2 and vGAT puncta, and co-localized GABA_AR γ2 and vGAT puncta densities (d). n = 23 (control) and 25 (S-SCAM) from triplicate experiments. Unpaired t test. F values are: GABA_AR γ2, F_(1,46) = 1.053, p = 0.8985; vGAT, F_(1,90) = 1.035, p = 0.9135 (b), GABA_AR γ2, F_(1,30.17) = 3.786, p = 0.0019; vGAT, F_(1,71) = 1.044, p = 0.8936 (c), GABA_AR γ2/vGAT, F_(1,54) = 1.335, p = 0.4577 (d). (e–j) Representative immunofluorescent images of GABA_AR α2 (e), NL2 (g), and gephyrin (i) in the dendrites of hippocampal neurons transfected with mCherry or myc-S-SCAM and quantification of puncta densities and average intensities of GABA_AR α2 (f), NL2 (h), and gephyrin (j). n = 23–26 per condition. Unpaired t test. F values are: GABA_AR α2 density, F_(1,44) = 2.162, p = 0.0773; intensity, F_(1,76) = 1.694, p = 0.1050 (f); NL2 density, F_(1,47) = 1.069, p = 0.8801; intensity, F_(1,70) = 1.542, p = 0.2035 (h); gephyrin density, F_(1,49) = 1.005, p = 0.9915, intensity, F_(1,76) = 1.544, p = 0.1852 (j). Scale bars, 5 μm.