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. 2020 Jan 9;10:83. doi: 10.1038/s41598-019-57053-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Excitatory synaptic targeting of S-SCAM deletion mutants and their effect on dendritic spines. (a) Schematic diagrams of S-SCAM deletion mutants. (b) Full-length expression of various S-SCAM deletion mutants expressed in COS-7 cells. Left panel, immunoblot probed with anti-myc antibody; right panel, Coomassie Blue stained blot. (c) Dendritic spine targeting of S-SCAM deletion mutants. Scale bars, 10 μm (low magnification images) or 5 μm (dendrites). (d) Quantified effect of S-SCAM deletion mutant overexpression on dendritic spine density. n = 42 (Control), 46 (WT), 41 (ΔGK), 41 (ΔWW), 39 (ΔGW), 45 (ΔPDZ5). One-way ANOVA, F_(5,248) = 41.86, p < 0.0001, Tukey’s post-hoc test. (e,f) Quantified effect of S-SCAM deletion mutant overexpression on dendritic spine width (e) and length (f). One-way ANOVA, F_(5,65) = 25.59, p < 0.0001 (e), F_(5,60) = 11.94, p < 0.0001 (f), Tukey’s post-hoc test.