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. 2020 Jan 9;10:83. doi: 10.1038/s41598-019-57053-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of S-SCAM deletion mutants on surface AMPA receptor levels. (a,b) Representative images of hippocampal neurons transfected with various S-SCAM deletion mutants stained for sGluA2 (a) and sGluA1(b). (c,d) Quantification of S-SCAM deletion mutant overexpression effect on the average intensities of sGluA2 (c) and sGluA1 (d). n = 27 and 47 (Control), 27 and 27 (WT), 28 and 23 (ΔGK), 27 and 20 (ΔWW), 23 and 29 (ΔGW), 24 and 20 (ΔPDZ5) for sGluA2 and sGluA1, respectively. One-way ANOVA, F_(5,150) = 43.63, p < 0.0001 (c), F_(5,160) = 71.15, p < 0.0001 (d), Tukey’s post-hoc test. Scale bars, 5 μm.