Skip to main content
. 2020 Jan 9;11:147. doi: 10.1038/s41467-019-13837-4

Fig. 6. Endolysosomal HIV-1 potentiates cytokine secretion from CD4+ T cells.

Fig. 6

CD4+ T cells were pre-treated with CXCR4 inhibitor AMD3100 or CCR5 inhibitor Maraviroc for 24 h, and TLR8-specific inhibitors CU-CPT9a, CU-CPT9b, or the negative control compound CU-CPT6 for 2 h, prior to concomitant TCR activation and co-culture with HEK293T cells expressing HIV-1-Gag-iGFP (X4 HIV-1) or HIV-Gag-iGFP JRFL (R5 HIV-1). Cytokines were analyzed in the supernatant 24 h post infection by multiplex ELISA. Results are presented as fold change relative to co-cultures in the absence of HIV-1. Bars represent means + SEM from 7 to 10 independent experiments with 5 μM inhibitor and three independent experiments with 25 μM inhibitor. Statistical significance (inhibitor samples versus untreated) was determined using repeated measures one-way ANOVA with Dunnettʼs post-test on log-transformed data; significance levels: *p < 0.05; **p < 0.01; ***p < 0.001. Source data are provided as a Source Data File.