Table 2.
Structural characteristics of independent Bvht modules.
| Samplea | 5′ module | 3′ module | Full-length | |||
|---|---|---|---|---|---|---|
| MW (kDa) | 30.4 | 68.6 | 206 | |||
| MgCl2 concentration | 6 mM | 12 mM | 6 mM | 12 mM | 6 mM | 12 mM |
| Rg (Å) | 42 ± 0.2 | 46 ± 0.2 | 61 ± 0.3 | 63 ± 0.4 | 99.53 ± 0.2 | 85 ± 0.7 |
| Dmax (Å) | 135 | 150 | 185 | 190 | 287 | 260 |
| χ2 | 1.2 | 1.2 | 1.2 | 1.2 | 1.3 | 1.18 |
| NSD | 0.85 ± 0.03 | 0.88 ± 0.02 | 1.06 ± 0.02 | 1.04 ± 0.03 | 1.21 ± 0.04 | 1.24 ± 0.04 |
| Resolution (Å) | 31.3 | 31.8 | 34.9–36.5 | 34.1–36.0 | 13.6–35.0 | 14.2–37.2 |
aCentral module (nucleotides # 87–305 with expected MW of 67.3 kDa) sample did not show mono-dispersity. Therefore, we did not process the data
5′ module encompasses nucleotides # 1–98, 3′ module encompasses nucleotides # 315–538, full-length encompasses nucleotides GG and # 1–634. See Table 1 for more information on SAXS data analysis