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. 2020 Jan 9;10:103. doi: 10.1038/s41598-019-57096-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Localization of FLAG::FCD-2 in the C. elegans germline. (a) Dissected gonads from FLAG::fcd-2 and FLAG::fcd-2 spo-11 transgenic hermaphrodite worms were co- stained with anti-FLAG antibody (green) and DAPI (blue). No signal was observed in absence of spo-11 gene. Bar, 10 μm. (b) Schematic representation of the C. elegans gonad arm. Regions 1 and 2 consist of mitotic nuclei, region 3 consists of meiotic nuclei in transition zone (leptotene and zygotene). The regions 4, 5 and 6 correspond to early, middle and late pachytene respectively. The region 7 consists of diplotene nuclei. (c) Histograms represent quantification of FLAG::FCD-2 foci in germline of FLAG::fcd-2 strain. The y-axis represents the percentage of nuclei with the indicated number of foci. The x-axis represents the position (zone) along the germline.