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. 2020 Jan 9;10:103. doi: 10.1038/s41598-019-57096-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Genetic interaction between FCD-2 and the BRC-1/BRD-1 complex. (a) Quantification of FLAG::FCD-2 foci in germline of FLAG::fcd-2 and FLAG::fcd-2; brc-1 brd-1 strains. The y-axis represents the average of FLAG::FCD-2 foci per nucleus. The x-axis represents the position (zone) along the germline. An average of 60 nuclei for each gonad region were scored for each genotype. Error bars correspond to SEM calculated from at least three independent experiments. (b) Representative images of the late pachytene nuclei in indicated genotypes co-stained with anti-GFP antibody (green), and DAPI (blue). Bar, 5μm. (c) Graphic representation of the percentage of developmental defects/hatched eggs in the indicated genotypes observed before and after 24–48 hrs treatment with 180 μM CDDP. Number of the developmental defects/hatched eggs observed: wt (N2) untreated = 0/2480; fcd-2 untreated = 15/2018; brc-1 bard-1 untreated = 0/3972; fcd-2; brc-1 bard-1 untreated = 5/1810; wt (N2) CDDP = 30/696; fcd-2 CDDP = 60/617; brc-1 bard-1 CDDP = 50/1390; fcd-2; brc-1 bard-1 CDDP = 38/328. Developmental defect phenotypes observed: dumpy, long, multivulva, roller, small, uncoordinated, larval arrest, vulvaless, vulva protrusion, bag of worm. Error bars correspond to SD calculated from at least three independent experiments. P value (x² test): n.s. = no significant difference. (d) Quantification of germline apoptosis in the indicated genotypes. The y-axis shows the average number of SYTO-12-labeled nuclei per gonadal arm. Number of observed gonad: wt: 84; fcd-2: 109; brc-1 brd-1: 73; fcd-2; brc-1 brd-1: 74. Error bars correspond to SD calculated from at least three independent experiments. P value (Student’s t-test): **P < 0.05; ***P < 0.0001.