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. 2020 Jan 9;10:70. doi: 10.1038/s41598-019-56953-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PCR to detect VGCC isoforms in mouse LMCs isolated from popliteal (A) or IALS (B). LMCs were collected by micropipette aspiration after isolation, cleaning and digestion of the respective vessels. Ca_v3.1 and Ca_v3.2, but not Ca_v3.3 isoforms are detected in LMCs from both vessels. Ca_v1.2 shows a strong band in both samples. eNOS is detected in cells collected from PLs (A) indicating that there is some contamination by endothelial cells (see Suppl. Fig. 1 for additional information). M = marker; bp = base pairs.