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. Author manuscript; available in PMC: 2021 Jan 8.
Published in final edited form as: Cell Host Microbe. 2019 Dec 19;27(1):104–114.e4. doi: 10.1016/j.chom.2019.11.013

Figure 6. HCMV miR-UL22A targeting of SMAD3 is essential for latency in CD34+ HPCs.

Figure 6.

A) NHDF were infected with WT, ΔmiR-UL22A or ΔmiR-UL22A/SMAD3shRNA and protein was harvested at the indicated timepoints and assessed for SMAD3, HCMV IE2 and GAPDH protein expression. B) CD34+ HPCs were infected with WT, ΔmiR-UL22A and ΔmiR-UL22A/SMAD3 shRNA viruses and analyzed as in Figure 5A to determine SERPINE transcript levels. Experiment was performed in duplicate. Error bars represent the standard error of the mean. Significance was determined by t-test. *p<0.05 C) CD34+ HPCs were infected with HCMV, HCMVΔmiR-UL22A and HCMVΔmiR-UL22A/SMAD3 shRNA and analyzed for latency and reactivation as described in Figure 1. Results from two representative donors are shown. D) HCMV genome copy number in initially sorted (2dpi) and latently-infected HPCs (14dpi) from both two donors was assessed by qPCR for HCMV UL141 and normalized to cellular β-globin. Error bars represent the standard error of the mean of three triplicate wells. See also Figure S2D.