KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-human TGF-beta 1, 2, 3 | R&D Systems | Cat# MAB1835 |
| Mouse monoclonal anti-human NAB1 | Santa Cruz Biotechnology | Cat# sc-81565 |
| Mouse monoclonal anti-human GAPDH | Abcam | Cat# ab8245 |
| Rabbit monoclonal anti-human SMAD3 | Abcam | Cat# ab40854 |
| Mouse monoclonal anti-HCMV IE2 | Sigma Aldrich | Cat# MAB810 |
| Bacterial and Virus Strains | ||
| HCMV BAC: TB40E-GFP | GenBank ref # EF999921.1 | |
| Biological Samples | ||
| Human: primary fetal liver, de-identified, 12-20 wks | Advanced Bioscience Resources | n/a |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Recombinant human TGF-β | Peprotech | Cat# 100-21 |
| Critical Commercial Assays | ||
| P3 primary cell nucleofection kit | Lonza | Cat# V4XP-3032 |
| Cytokine Human Magnetic 30-plex panel | ThermoFisher | Cat# LHC6003MCytokine |
| LegendMAX total TGF-β1 ELISA | Biolegend | Cat# 436707 |
| SMAD3 Taqman Assay | ThermoFisher | Hs00969210_m1 |
| SERPINE Taqman Assay | ThermoFisher | Hs00167155_m1 |
| 18S rRNA Tarman Assay | ThermoFisher | Hs03928990_g1 |
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| Human: NHDF | ATCC | Cat# PCS-201-012 |
| Human: HEK293T | ATCC | Cat# CRL-11268 |
| Human: Kasumi-3 | ATCC | Cat# CRL-2725 |
| Human: AEC | Lonza | CC-2535 |
| Mouse: M2-10B4 | Stem Cell Technologies | Cat# 00301 |
| Mouse: S1/S1 | Stem Cell Technologies | Cat# 00302 |
| Experimental Models: Organisms/Strains | ||
| Oligonucleotides | ||
| miR-US5-2 galk F primer: ACGAGAGCGTTCATCGGGGCATGAAGTACGCATTACACAAACTCCATATATTTGTTACGATAGAATACGGAACGGACCTGTTGACAATTAATCATCGGCA | This study | n/a |
| miR-US5-2 galk R primer: TATGCACAAAAGGTATGTGTGAATGGAAATACATGATGAATGTCATCATCACGCAAAGCAGCCGTGGGAATGGTCTCAGCAAAAGTTCGATTTATTCAAC | This study | n/a |
| miR-US5-2 F primer: TACGCATTACACAAACTCCATATATTTGTTACGATAGAATACGGAACGGAACCATTCCCACGGCTGCTTTGCGTGATGATGACATTCATCATGTATTTCC | This study | n/a |
| miR-US5-2 R primer: GGAAATACATGATGAATGTCATCATCACGCAAAGCAGCCGTGGGAATGGTTCCGTTCCGTATTCTATCGTAACAAATATATGGAGTTTGTGTAATGCGTA | This study | n/a |
| NAB1 3’ UTR F primer: GAGCCTGAAGATTCAAGATAG | This study | n/a |
| NAB1 3’ UTR R primer: GTGAGGCACAGAAAATCTTTATTG | This study | n/a |
| miR-US5-2 NAB1 binding site 1, F primer: GATTTAAGGATAATACTCCGAAATAAGCCTTAATAACC | This study | n/a |
| miR-US5-2 NAB1 binding site 1, R primer: GGTTATTAAGGCTTATTTCGGAGTATTATCCTTAAATC | This study | n/a |
| miR-US5-2 NAB1 binding site 2, F primer: AGTTCTGCTCTAGTCATAATTGTAA | This study | n/a |
| miR-US5-2 NAB1 binding site 2, R primer: TTACAATTATGACTAGAGCAGAACT | This study | n/a |
| Please see Table S2 for additional oligonucleotide sequences | ||
| Recombinant DNA | ||
| pRL SV40-Rluc | Promega | Cat# E2231 |
| pSiCheck2 | Promega | PR-C8021 |
| pSIREN-GFP | Clontech | Cat# 632455 |
| SMAD Cignal Reporter Assay | Qiagen | Cat# CCS-017G |
| TGF-β siRNA | ThermoFisher | Cat # 4427038 Assay ID: s14054 |
| NAB1 siRNA | ThermoFisher | Cat # 4427037 Assay ID: s9245 |
| SMAD3 siRNA | ThermoFisher | Cat # 4392420 Assay ID: s535082 |
| RISC-free siRNA negative control | ThermoFisher | Cat# D-001220-01-05 |
| Software and Algorithms | ||
| ImageJ | Schneider et al., 2012 | https://imagej.nih.gov/ij/ |
| FACS Diva | BD | |
| Prism (v6 or v7) | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| Other | ||