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. Author manuscript; available in PMC: 2020 Jul 6.
Published in final edited form as: Nat Cell Biol. 2020 Jan 6;22(1):97–107. doi: 10.1038/s41556-019-0443-x

Extended Data Fig. 7. Inhibition of multicellular networks and diffuse infiltration into mouse brain after p120 downregulation.

Extended Data Fig. 7

a, Impaired tumor growth, loss of network formation and diffuse brain infiltration of E-98 cells after p120 downregulation. Overviews (upper panels) and zoom images (lower panels) from 200 μm thick brain slices. Arrowheads, filaments in multicellular networks of human vimentin-positive E-98 cells diffusely infiltrating the brain. Data represent 9 (NT shRNA) or 10 (p120 shRNA) mice from 2 independent experimental series (a1) and 49 cells (NT shRNA) or 89 (p120 shRNA) cells from two mice per condition (a2). P values, two-tailed Mann-Whitney test. b, Persistent deficiency of p120 signal in vivo in p120 shRNA knock-down E-98 cells in 4 weeks after intracranial tumor implantation. Similar results were observed in two mice per condition. c, Sustained glioma growth deficit in vivo after p120 downregulation. Frequency of geminin-positive glioma cells after the indicated time of post-implantation in mouse brain. Data represent 42–201 (E-98) and 108–574 (E-478) cells from 4–5 (E-98) or 5–21 (E-468) imaging fields from two mice per condition. P values, two-tailed Mann-Whitney test. Scatter dot plots show the medians (red line) and values representing individual mice (a1), individual cells (a2) or imaged areas in brain slices (c). Scale bars, 2 mm (a, upper panels), 50 μm (a, lower panels; b, c).