Fig. 3.

GCN2 promotes antigen-specific CD8⁺ T-cell responses against glioma. In a, WT OT-1 and GCN2 KO OT-1 mice were challenged directly with 4 × 10⁵ GL-261 OVA and were analyzed for survival. In b, OVA activated CD8⁺ T-cells from WT OT-1 or GCN2 KO OT-1 mice were transferred i.c. into tumor-bearing Rag1 KO hosts and analyzed for survival. In c, OVA activated CD8⁺ T-cells from WT OT-1 and GCN2 KO OT-1 mice were isolated and labeled with cell proliferation dye eFluor 450 (GCN2 KO CD8⁺ T-cells) and cell proliferation dye eFluor 670 (WT OT-1 CD8⁺ T-cells), and were transferred at a 1:1 ratio into GL-261 OVA tumor-bearing mice either i.c. (left panel) or i.v. (right panel). After 3 days, mice were euthanized for flow cytometric analysis. In a, b, Kaplan–Meier curves were generated from n = 7 mice per group from two independent experiments, and statistical significance calculated using the Log-Rank analysis. In c, flow cytometry statistics calculated from n = 3 mice per condition. Unpaired T test analysis was used to calculate significance. p < 0.05*; p < 0.01**