Fig. 4.

Inhibition of memory and function of in vivo activated CD8⁺ T-cells with GCN2 deletion. In a, b, 1 × 10⁶ heat shock killed GL-261 OVA cells were injected into OT-1 and GCN2 KO OT-1 mice. After 14 days, CD8⁺ T-cells from these mice were isolated and transferred i.v. into previously inoculated Rag1 KO mice (4 × 10⁵ GL-261 OVA). The first group of mice was analyzed for survival analysis (a) and the second group of mice was euthanized 7 days post-transfer for flow cytometric analysis (b). In a, the Kaplan–Meier curve was generated from n = 7 per group from two independent experiments, and statistical significance calculated using the Log-Rank analysis. In b, flow cytometry statistics calculated and shown as the total number and percentage positive population, n = 3 per group, representative of two experiments. Unpaired T test analysis was used to calculate significance. p < 0.05*; p < 0.01**. Refer to Supplementary Fig. 7c for the gating strategy