Table 1.

EC₅₀ values of the double modified CSP2 analogs against the ComD1 and ComD2 receptors^a

			ComD1		ComD2
Peptide Number	Peptide Name	Sequence	EC50 (nM)b	95% CIc	EC50 (nM)b	95% CIc
	CSP1e	EMRLSKFFRDFILQRKK	10.3	6.27–16.8	526	498–556
	CSP2e	EMRISRIILDFLFLRKK	1650	1190–2300	50.7	40.6–63.2
	CSP2(15)f	EMRISRIILDFLFLR	--d	--	21.9	11.5–41.6
1	CSP2(15)-I4LI7F	EMRLSRFILDFLFLR	--d	--	>1000	--
2	CSP2(15)-I4LL9R	EMRLSRIIRDFLFLR	685	469–999	58.2	42.3–80.1
3	CSP2(15)-I4LL12I	EMRLSRIILDFIFLR	--d	--	19.6	8.60–44.8
4	CSP2(15)-I4LF13L	EMRLSRIILDFLLLR	610	414–897	9.16	7.49–11.2
5	CSP2(15)-I4LL14Q	EMRLSRIILDFLFQR	--d	--	5.51	2.31–13.1
6	CSP2(15)-I7FL9R	EMRISRFIRDFLFLR	661	304–1437	149	73.7–304
7	CSP2(15)-I7FL12I	EMRISRFILDFIFLR	--d	--	26.3	20.5–33.8
8	CSP2(15)-I7FF13L	EMRISRFILDFLLLR	--d	--	67.2	30.6–147
9	CSP2(15)-I7FL14Q	EMRISRFILDFLFQR	--d	--	82.6	53.0–128
10	CSP2(15)-L9RL12I	EMRISRIIRDFIFLR	--d	--	77.6	45.4–132
11	CSP2(15)-L9RF13L	EMRISRIIRDFLLLR	--d	--	78.1	46.6–130
12	CSP2(15)-L9RL14Q	EMRISRIIRDFLFQR	408	361–462	15.2	6.14–37.5
13	CSP2(15)-L12IF13L	EMRISRIILDFILLR	--d	--	44.5	22.5–88.0
14	CSP2(15)-L12IL14Q	EMRISRIILDFIFQR	--d	--	72.2	49.6–104
15	CSP2(15)-F13LL14Q	EMRISRIILDFLLQR	--d	--	22.5	19.2–26.2

^aSee experimental section for detail of reporter strains. See supporting information for methods and plots of agonism dose response curves. All assays were performed in triplicates.

^bEC₅₀ values were determined by testing peptides over a range of concentrations.

^c95% confidence interval.

^dEC₅₀ not determined due to the analog’s low induction in primary agonism screening assay.

^eData from ref. 28.

^fData from ref. 20.