. Author manuscript; available in PMC: 2021 Feb 3.

Published in final edited form as: Chembiochem. 2019 Oct 15;21(3):340–345. doi: 10.1002/cbic.201900365

Table 3.

EC₅₀ values of the quintuple modified CSP2 analogs against the ComD1 and ComD2 receptors^a

			ComD1		ComD2
Peptide Number	Peptide Name	Sequence	EC₅₀ (nM)^b	95% CI^c	EC₅₀ (nM)^b	95% CI^c
21	CSP2(15)-I4LR6KL9RF13LL14Q	EMRLSKIIRDFLLQR	>1000	--	141	85.5–235
22	CSP2(15)-I4LI7FL9RF13LL14Q	EMRLSRFIRDFLLQR	266	144–492	>1000	--
23	CSP2(15)-I4LI8FL9RF13LL14Q	EMRLSRIFRDFLLQR	75.3	59.4–95.4	41.0	21.2–79.0
24	CSP2(15)-I4LL9RL12IF13LL14Q	EMRLSRIIRDFILQR	>1000	--	158	64.5–387

See experimental section for detail of reporter strains. See supporting information for methods and plots of agonism dose response curves. All assays were performed in triplicates.

EC₅₀ values were determined by testing peptides over a range of concentrations.

95% confidence interval.