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Thermal stability of ICab3 and its mutants. Nano-DSF profiles of WT ICab3 and the mutants showing highest thermal stability for WT ICab3 (A) followed by ICab3 V115C (B) and ICab3 H116C (C). The Tm of WT ICab3 resembles that of ICab3 H116C upon the addition of 0.5 mm EDTA. While, Tm of WT ICab3 increases upon the addition of Zn2+, neither its excess nor the presence of EDTA as its chelator alters the Tm of ICab3 V115C or ICab3 H116C.
