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. 2019 Nov 18;295(1):69–82. doi: 10.1074/jbc.RA119.009548

Figure 1.

Figure 1.

Regulatory genes of ferroptosis are up-regulated with decreased BACH1 protein in the induction of ferroptosis. A, RNA-seq was performed in WT MEFs (9th passage: P9) with only DMSO (DMSO group) or DMSO + 3 μm erastin (Erastin group) for 24 h. A heat map of gene expression profiles shows the genes registered to map04216 (Ferroptosis pathway) of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway map. The genes were arranged from the bottom in the order of fold-change of erastin group to DMSO group. n = 3 per group. B and C, Western blotting for BACH1, HO-1, and GAPDH of WT MEFs (7th passage: P7, 11th passage: P11) exposed to erastin for 12 h. Representative image (B) and the intensity of bands (C). D and E, Western blotting for BACH1 and GAPDH in WT MEFs (8th passage) exposed to erastin and 25 μm MG132 for 6 h. Representative image (D) and the intensity of bands (E). In A, p value by the differential expression analysis performed on edge R. In C and E, error bars represent S.D. p value by Tukey's test after two-way ANOVA.