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. 2019 Dec 4;8(12):1565. doi: 10.3390/cells8121565

Figure 3.

Figure 3

Myoblast differentiation following BSA treatment. Cells were cultured in serum-free media supplemented with T4 or T4 + BSA for two days (A–E). (A) Myotube formation and fusion index were observed by Giemsa staining. mRNA level was observed by real-time RT-PCR and protein expressions by Western blot and immunocytochemistry. (B) TTR mRNA in exosomes of cultured media using RT-PCR and protein level in cultured media by Western blot. (C) T4 or T3 concentration in cultured media or cells was observed by ELISA. (D,E) Cells were cultured with serum-free media supplemented with T4, T4 + BSA, T4 + TTR or T4 + BSA + TTR for two days. T4 or T3 concentration in T4 + BSA or T4 + BSA + TTR treated cells. TTR mRNA in exosomes of cultured media (in T4 + BSA or T4 + BSA + TTR treated cells) using RT-PCR. (F) Cells were cultured in serum-free media supplemented with T4 or T4 + BSA or T4 + TTR for two days and exosomes were isolated from each cultured medium. T3 concentration in exosomes. Means ± SD (n = 3). * p ≤ 0.05, ** p ≤ 0.001, *** p ≤ 0.0001.