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. 2019 Nov 23;8(12):1502. doi: 10.3390/cells8121502

Figure 5.

Figure 5

Translocation of SOD1G93A into nuclei by overexpressing PDI in primary cultured neurons. (a) Interaction between SOD1G93A and PDI. SOD1WT and SOD1G93A interaction with PDI in the yeast two-hybrid system. (b) Plasmid constructed for co-expressing SOD1G93A-GFP and PDI-mCherry. (c) Nucleic localization of SOD1G93A with PDI. The co-expressed PDI-mCherry (red) enhanced the nucleic localization of SOD1G93A-GFP (green) in primary cultured neurons (upper). Both SOD1G93A-GFP and PDI-mCherry proteins were colocalized in the ER, whereas, only SOD1G93A-GFP was detected in the nuclei (lower). (scale bar is 20 μm). (d) Statistical analysis of nucleic localization of SOD1G93A-GFP in primary cultured WT and SOD1G93A neurons (results in triplicates); Dark gray color: Only SOD1G93A-GFP expression; weak gray color: Co-expression of SOD1G93A-GFP and PDI-mCherry. (n = 116 for WT neurons and 157 for SOD1G93A background neurons, error bars: Standard deviation).