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. 2019 Nov 30;8(12):1551. doi: 10.3390/cells8121551

Figure 1.

Figure 1

Generation of neurospheres from the ventricular-subventricular zone (V-SVZ) of young mice. (A) Photomicrographs showing floating neurospheres obtained from wild-type mice. (B) Representative bands for angiotensinogen (ANG), Ang II type-1 (AT1) and type-2 (AT2) receptors, and β-actin obtained by RT-PCR in neurospheres (NF). Homogenates of striatum (ST) were used as a positive control. (C) ANG was detected in neurosphere culture medium by HPLC and visualized by western blot. ANG (250 μg/mL) was used as a positive control (C+). Bar graphs showing the number (D) and diameter (E) of neurospheres after treatment with angiotensin II (Ang II), AT1 receptor antagonist (ZD7155 or candesartan), and AT2 receptor antagonist (PD123319). Histograms showing the number of neurospheres after treatment with AT2 receptor agonists (CGP42112A or C21) and the AT2 antagonist PD123319 ((F); treatment) or in cultures derived from neurospheres pre-treated with AT2 agonists and reseeded in the absence of any treatment ((G); neurospheres derived from cultures pre-treated with AT2 agonists; pre- = previously treated with). All culture data were obtained from at least three separated experiments. Data are means ± standard error of the mean (SEM). * p < 0.05 relative to control (untreated) group (one-way ANOVA and Bonferroni post hoc test.). SEM = standard error of the mean. ANOVA = analysis of variance. bp = base pairs. Scale bar: 150 μm.