Table 1.
Sequences of forward and reverse primers for the desired target organisms.
| Target Organism | Primer | Sequence (5ʹ-3ʹ) | PCR Product Size (bp) |
|---|---|---|---|
| Bacteroidales | GenbacIII-F GenbacIII-R |
GGGGTTCTGAGAGGAAGGT CCGTCATCCTTCACGCTACT |
129 |
|
Escherichia coli
(E. coli) |
H8-F H8-R |
ACAGTCAGCGAGATTCTTC GAACGTCAGCACCACCAA |
177 |
| E. coli | UidA-F UidA-R |
CGGAAGCAACGCGTAAACTC GAGCGTCGCAGAACATTACATT |
68 |
Ethical approval for the study of the effectiveness of molecular assays in detecting human faecal pollution (4 February 2013), and the effect of freezer storage on human faecal samples was done by the ethics committee through Griffith University office of research (Reference No. BPS/01/13/HREC, 4 February 2013). Written informed consent was obtained from all participants.