Figure 7.

Detection of global and WRKY53-locus-specific H3K4 methylations, H3K9 acetylation, and RNAP II recruitment in 28-day-old plants at 4 h post-treatment with H₂O or H₂O₂. (a) Global immunodetection on isolated total proteins using indicated specific antibodies; (b) Schematic dispatch of the promoter and 5′-UTR region in WRKY53 locus, showing the location of the two detection PCR amplicons (I and II); (c) ChIP-PCR determination of relative enrichment of DNA at I and II of the WRKY53 locus, shown as normalized ratios of ChIP/input to that of the H₂O treatments (mock, in three biological replicates). Individual data for three biological replicates normalized to their respective mock treatments were plotted, with minimum to maximum bars and the medium points in red. Asterisks indicate significant differences from mock using a paired Student’s t-test. * p < 0.05; ** p < 0.01.