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. 2019 Nov 23;8(12):1499. doi: 10.3390/cells8121499

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of IGF1R/INSR silencing on cell proliferation. IGF1R-KD, INSR-KD and control MCF7 cells (A) and IGF1R siRNA-, INSR siRNA- or control siRNA-transfected T47D cells (B) were plated in 96-well plates. After 24 h, the medium was replaced with starvation media in the absence (solid bars) or presence of IGF1 (cross-hatched bars) or insulin (squares-filled bars) (50 ng/mL) for an additional 72 h (MCF7) or 48 h (T47D), after which cell viability was determined by an XTT assay. A value of 100% was given to the cell number of control, untreated cells at the end of the incubation period. *, p < 0.05 IGF1-treated versus control cells; ^§, p < 0.05 insulin-treated versus control cells. All experiments were conducted in triplicates.