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. 2019 Dec 3;8(12):1557. doi: 10.3390/cells8121557

Figure 2.

Figure 2

Expression of KRAS mutants enhanced the motility of NIH3T3 cells. (A,B) Representative micrographs of NIH3T3 cells transfected with (A) KRAS and (B) NRAS constructs after scratching the cell monolayers (0 h) and at 20 h post-scratch. Scale bars: 100 µm. (C,D) Percent open wound of the field view area occupied by (C) KRAS and (D) NRAS overexpressing NIH3T3 cells at 20 h post-scratch vs. time point 0 h. (E–J) Western blotting detection of epithelial-mesenchymal transition (EMT) markers (F,I) E-cadherin and (G,J) vimentin protein expression levels in NIH3T3 cells transfected with KRAS or NRAS expression constructs. Band intensities were normalized against GAPDH or total protein. Data presented are representative of three independent trials in triplicates and expressed as mean (standard deviation). ** P ≤ 0.01, *** P ≤ 0.001. EMT, epithelial-to-mesenchymal transition.