Skip to main content
. 2019 Dec 3;8(12):1557. doi: 10.3390/cells8121557

Figure 4.

Figure 4

Expression of KRAS and NRAS mutants altered the gross cellular morphology of NIH3T3 cells. (A,B) Representative micrographs showing the cellular morphology of NIH3T3 cells co-transfected with empty vector (i.e., pTargeT) and (A) KRAS or (B) NRAS expression constructs. Cells were co-transfected with empty pmR-ZsGreen1 to estimate transfection efficiency. Arrows indicate cells with smaller, rounder, and more refringent morphology in contrast to empty vector and wild-type control cells which are flatter and more spread out. Scale bars: 25 µm. (C,D) Quantification of NIH3T3 cells overexpressing (C) KRAS and (D) NRAS mutants exhibiting apparent morphological alterations such as decreased size, refringency, and increased cellular protrusions. Data presented are representative of three independent trials and expressed as mean (standard deviation). ** P ≤ 0.01, *** P ≤ 0.001.