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. 2019 Nov 21;8(12):1481. doi: 10.3390/cells8121481

Figure 7.

Figure 7

Effects of cJun dimerization-specific, and phosphorylation-deficient and -mimicking mutants on the expression of TGFβ -Smad and AP-1 target genes. (a,b) Differential effects of cJun-m2,5-6D or cJun-m2,5-7A on TGFβ-inducible gene expression. MCF10A MII cells stably overexpressing cJun-m2,5-6D or cJun-m2,5-7A were incubated in EGF-containing starvation medium for 16 h and treated, or not, with TGFβ (5 ng/mL) for 16 h, after which mRNA levels were analyzed by qPCR and normalized to GAPDH. In panel (b) the ratio of the expression in cJun-m2,5-7A cells versus cJun-m2,5-6D cells is depicted. (c) Immunoblot analysis of MCF10A MII cells overexpressing cJun-m2,5-6D or cJun-m2,5-7A, treated with TGFβ (5 ng/mL) or mock-treated, as indicated. (d) Analysis of 166 estrogen-receptor-positive (ER+) human breast cancer cases showing enhanced levels of wnt7b in breast cancers cells with loss-of-function mutations in the upstream JNK pathway components MAP2K4 and MAP3K1.