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. 2019 Dec 11;8(12):1613. doi: 10.3390/cells8121613

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Characterization of cardiac-induced cells: (A) Analysis of mRNA expression after six days of cardiac differentiation shows a decrease of the pluripotency marker Oct4, whereas cardiac markers are upregulated. The mRNA expression was normalized to the expression of the house keeping gene hprt. Values are expressed as fold increase as compared with day 0. Values are presented as mean ± SEM. (B) Starting at day 7 of the cardiac differentiation protocol, beating foci per embryoid bodies (EB) were counted until the end of differentiation (n = 3). Values are presented as mean ± SD. p-value was calculated using the student t-test.