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Schematic of self-assembling scaffolds. (A) Self-assembly of functionalized polymers, 8-arm polyethylene glycol functionalized with thiol (PEG-8-SH) and acrylate (PEG-8-Acr) via a thiol–Michael addition reaction. (B) The encapsulation of H9 cells human embryonic stem cells (ESCs), was achieved upon mixing with the self-assembling polymers in a syringe mold. Following polymerization, the scaffolds were then incubated in culture plates containing medium.
