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. 2019 Dec 16;8(12):1650. doi: 10.3390/cells8121650

Figure 3.

Figure 3

Self-renewal and pluripotency of ESCs were maintained following extended culture in 3-D scaffolds. (A–C) Cell morphology of ESCs (H9 cells) grown in 2-D cultures prior to encapsulation, in self-assembling scaffolds for 3-D culture for 21 days, and then subsequently subcultured back to 2-D culture conditions, respectively, as determined by light microscopy. All scale bars represent 100 μm. (D) Comparison of expression of OCT4, NANOG, and SOX2 in ESCs grown in 2-D conditions and first in 3-D self-assembling scaffolds, and then passaged in 2-D culture conditions as determined by qRT-PCR. Results were expressed as the fold expression ± SE normalized to reference genes HMBS, GAPDH, and β-ACTIN (* p < 0.05 and ** p < 0.01).