Table 1.
List of human primer sequences used in qRT-PCR.
| Gene | Primer Sequence | ||
|---|---|---|---|
| Forward (5′-3′) | Reverse (5′-3′) | Product Length | |
| ACTIN | AATCTGGCACCACACCTTCTAC | ATAGCACAGCCTGGATAGCAAC | 170 |
| BRACHYURY | TGCTTCCCTGAGACCCAGTT | GATCACTTCTTTCCTTTGCATCAAG | 121 |
| DNMT3L | CTGCTCCATCTGCTGCTCC | ATCCACACACTCGAAGCAGT | 85 |
| DPPA3 | AGACCAACAAACAAGGAGCCT | CCCATCCATTAGACACGCAGA | 88 |
| ESRRB | GACATTGCCTCTGGCTACCA | CTCCGTTTGGTGATCTCGCA | 131 |
| FGF4 | CGTGGTGAGCATCTTCGGC | GTAGGACTCGTAGGCGTTGT | 145 |
| FOXA2 | GGGAGCGGTGAAGATGGA | TCATGTTGCTCACGGAGGAGTA | 89 |
| FOXF1 | AAGCCGCCCTATTCCTACATC | GCGCTTGGTGGGTGAACT | 63 |
| GAPDH | ACAACTTTGGTATCGTGGAAGG | GCCATCACGCCACAGTTTC | 101 |
| GATA4 | TCCCTCTTCCCTCCTCAAAT | TCAGCGTGTAAAGGCATCTG | 194 |
| GATA6 | CCCACAACACAACCTACAGC | GCGAGACTGACGCCTATGTA | 131 |
| GDF3 | GTCTCCCGAGACTTATGCTACG | AGTAGAGGAGCTTCTGCAGGCA | 136 |
| GP130 | GGAGTGAAGAAGCAAGTGGGA | AGGCAATGTCTTCCACACGA | 128 |
| HMBS | AGGAGTTCAGTGCCATCATCCT | CACAGCATACATGCATTCCTCA | 104 |
| ITGA2 | TTGCGTGTGGACATCAGTCT | GCTGGTATTTGTCGGACATCT | 158 |
| ITGA5 | GCCGATTCACATCGCTCTCAA | GTCTTCTCCACAGTCCAGCAA | 139 |
| ITGA6 | CGAAACCAAGGTTCTGAGCCC | CTTGGATCTCCACTGAGGCAG | 151 |
| ITGAV | AGGAGAAGGTGCCTACGAAGC | GCACAGGAAAGTCTTGCTAAGG | 105 |
| ITGB1 | GGATTCTCCAGAAGGTGGTTT | TGCCACCAAGTTTCCCATCT | 143 |
| ITGB3 | CATGGATTCCAGCAATGTCCTC | TTGAGGCAGGTGGCATTGAAG | 126 |
| ITGB5 | GCCTTTCTGTGAGTGCGACAA | CCGATGTAACCTGCATGGCAC | 111 |
| KLF17 | TCAGGAAGGGACTGGTAGAA | GTACCCGCATATGTCGTCTAAG | 206 |
| KLF2 | CCAAGAGTTCGCATCTGAAGG | CCGTGTGCTTTCGGTAGTG | 132 |
| KLF4 | CGAACCCACACAGGTGAGAA | TACGGTAGTGCCTGGTCAGTTC | 75 |
| KLF5 | ACCCTGGTTGCACAAAAGTT | CAGCCTTCCCAGGTACACTT | 100 |
| LATS1 | CTCTGCACTGGCTTCAGATG | TCCGCTCTAATGGCTTCAGT | 145 |
| LATS2 | ACATTCACTGGTGGGGACTC | GTGGGAGTAGGTGCCAAAAA | 147 |
| LIFR | CACCTTCCAAAATAGCGAGTATGG | ATGGTTCCGACCGAGACGAGTT | 159 |
| MIXL1 | CCGAGTCCAGGATCCAGGTA | CTCTGACGCCGAGACTTGG | 58 |
| NANOG | AAAGAATCTTCACCTATGCC | GAAGGAAGAGGAGAGACAGT | 110 |
| N-CADHERIN | TGTTTGGCCTGGCGTTCTTT | AGGAGACAGAAACGAAGCCA | 156 |
| NCAM | AGGAGACAGAAACGAAGCCA | GGTGTTGGAAATGCTCTGGT | 161 |
| OCT4 | CCCCTGGTGCCGTGAA | GCAAATTGCTCGAGTTCTTTCTG | 97 |
| PAX6 | CTTTGCTTGGGAAATCCGAG | AGCCAGGTTGCGAAGAACTC | 103 |
| PRDM14 | CCTTGTGTGGTATGGAGACTGC | CTTTCACATCTGTAGCCTTCTGC | 126 |
| RAC1 | ATGTCCGTGCAAAGTGGTATC | CTCGGATCGCTTCGTCAAACA | 249 |
| RHOA | CATCCGGAAGAAACTGGT | TCCCACAAAGCCAACTC | 168 |
| ROCK1 | GGTGGTCGGTTGGGGTATTTT | CGCCCTAACCTCACTTCCC | 196 |
| SALL2 | GGCTTGCCTTATGGTATGTCCG | TGGCACTGAGTGCTGTTGTGGA | 115 |
| SOCS3 | ATTCGGGACCAGCCCCC | AAACTTGCTGTGGGTGACCA | 121 |
| SOX11 | CGACGACCTAATGTTCGACC | GACAGGGATAGGTTCCCCG | 105 |
| SOX17 | CGCACGGAATTTGAACAGTA | GGATCAGGGACCTGTCACAC | 182 |
| SOX2 | TTGCTGCCTCTTTAAGACTAGGA | CTGGGGCTCAAACTTCTCTC | 75 |
| SOX7 | ACGCCGAGCTCAGCAAGAT | TCCACGTACGGCCTCTTCTG | 73 |
| STAT3 | CTTTGAGACCGAGGTGTATCAC | GGTCAGCATGTTGTACCACAG | 133 |
| TAZ | GAGGGTGTATGGTGGAGATAAA | CCAACTGTAGCAAACAGGATTAG | 86 |
| TBX3 | GGACACTGGAAATGGCCGAAG | GCTGCTTGTTCACTGGAGGAC | 123 |
| TBX3 | CGGACATACTTGTTCCCCGA | GCAGGGTGAGCTGTTTTCTTTT | 154 |
| TEAD4 | CCAAGCTCTGGATGTTGGAGTTC | GATGTCCACGGCTTCGAGGTA | 161 |
| TFCP2L1 | TTTGTGGGACCCTGCGAAG | TGCTTAAACGTGTCAATCTGGA | 129 |
| TUJ1 | GGCCAAGTTCTGGGAAGTCA | CGAGTCGCCCACGTAGTTG | 70 |
| YAP | GCTGCCACCAAGCTAGATAA | GTGCATGTGTCTCCTTAGATCC | 101 |
| ZFP42 | CGCAATCGCTTGTCCTCAGA | GCTCTCAACGAACGCTTTCC | 130 |
| ZIC2 | CGCTCCGAGAACCTCAAGAT | CCCTCAAACTCACACTGGAA | 71 |