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. 2019 Dec 7;8(12):1592. doi: 10.3390/cells8121592

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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qPCR analyses of miRNAs identified by deep sequencing as being responsive to ASAL alone (n = 8–9). Average log₂ RQs with SE bars are plotted. An asterisk (*) represents a significant difference between diets in each injection treatment (p < 0.05) with fold-change given in brackets. A dagger (†) represents a significant difference between PAMP-injected salmon and the diet-matched PBS-injected control (p < 0.05) with fold-change indicated below the x-axis. For qPCR fold-change calculation, overall fold up-regulation was calculated as 2^A−B as in Xue et al. [46], where A is the mean of log₂ RQ from the pIC or ASAL groups, and B is the mean of log₂ RQ from the diet-matched PBS group. For down-regulated miRNAs, fold-change values were inverted (−1/fold-change). (A) miR-192a-5p; (B) miR-194a-5p; (C) miR-725-5p; (D) miR-novel-16-5p; (E) miR-722-3p; (F) miR-727a-3p.