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. 2019 Dec 7;8(12):1592. doi: 10.3390/cells8121592

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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qPCR analyses of basal expression (pre-injection samples) of candidate pIC- and/or ASAL-responsive miRNAs identified by deep sequencing (n = 8). Average log₂ RQs with SE bars are plotted. An asterisk (*) indicates a significant difference between diets for a given miRNA (p < 0.05) with fold-change given in brackets. For qPCR fold-change calculation, overall fold up-regulation was calculated as 2^A−B as in Xue et al. [46], where A is the mean of log₂ RQ from the CpG group, and B is the mean of log₂ RQ from the control group. For down-regulated miRNAs, fold-change values were inverted (−1/fold-change). (A) miR-27d-1-2-5p; (B) miR-29b-2-5p; (C) miR-30e-1-2-3p; (D) miR-135bd-5p; (E) miR-146a-5p; (F) miR-146a-1-2-3p; (G) miR-181a-5-3p; (H) miR-192a-5p; (I) miR-194a-5p; (J) miR-221-5p; (K) miR-462a-3p; (L) miR-722-3p; (M) miR-725-5p; (N) miR-727a-3p; (O) miR-novel-16-5p.