Figure 1.

Release of neutrophil extracellular traps (NETs) by quorum sensing molecule (QSM)-treated neutrophils. Neutrophils were treated with QSMs at variable concentrations for 3 h. Unstimulated cells served as a negative control. (a) The released NETs were digested with micrococcal nuclease (MNase) and collected supernatants were stained with Sytox Green and the fluorescence intensity was measured. Data represent the mean fluorescence ± standard error of the mean (S.E.M.). from two replicates. ANOVA and Dunnett’s multiple comparisons post-tests were used. Asterisks denote statistical significance (p > 0.1234 ns, * p ≤ 0.0332, **** p < 0.0001). (b) The extracellular DNA was stained with Sytox Green and visualized with fluorescence microscopy.