Figure 6.
Effect of cobalamin deficiency on redox homeostasis in cultured astrocytes. The analyses were performed for cobalamin-deficient (Cbl-) and control astrocytes. (a) Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activity was determined using specific colorimetric assays. (b) Intracellular reactive oxygen species (ROS) level evaluated by the use of CM-H2DCFDA probe. (c) Reduced thiol level determined following the staining of cells with VitaBright 48 (VB-48); the presented histograms are representative of three independent experiments; M1—cells with high level of reduced thiols. Bar graphs represent mean values ± SD of three independent experiments where the analysis was performed in at least three replicates; * p < 0.05 (unpaired t-test).