Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 15;8(12):1642. doi: 10.3390/cells8121642

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

END and ENL induce nuclear translocation of estrogen receptor (ER) and increase the expression of ER-responsive genes. (A,B) MCF7 cells were treated with 1 μM END and 1 μM ENL for different time intervals as indicated and localization of ER was examined using immunocytochemistry. (C) MCF7 cells were treated with 1 μM END, 1 μM ENL, and 100 nM E2 followed by nuclear and cytoplasmic extraction and immunoblot analyses for ER expression. (D,E) MCF7 cells were treated with 1 μM END, 1 μM ENL, and 100 nM E2 for indicated time intervals. Protein lysates and total RNA were isolated and examined for the expression of ER-responsive genes as noted.