Impaired secretion of galectin-3 and kynurenines by ASCs of patients with rheumatic diseases (RD). Five healthy donor (HD) ASCs lines from two different passages (n = 10), systemic lupus erythematosus (SLE; n = 16), systemic sclerosis (SSc; n = 16–17) and ankylosing spondylitis (AS; n = 15) patients’ ASCs were cultured in medium alone (C, control), or in the presence of the indicated cytokines (TI, TNF-α + IFN-γ). Galectin-3 and kynurenine concentrations were measured in culture supernatants. (A,B) Data are expressed as Tukey’s boxes (see Figure 1); * p = 0.05–0.01, *** p = 0.001–0.0001 for control versus cytokine-treated cells; #
p = 0.05–0.01, ##
p = 0.01–0.001, ###
p = 0.001–0.0001, ####
p < 0.0001 for HD/ASCs versus RD/ASCs comparisons. (C,D) Rs, Spearman’s rank correlation coefficient. Hollow circles represents RD/ASC samples, solid circle represents SSc/ASC samples. ESR - erythrocyte sedimentation rate.