Figure 3.

Expression and localization of RITA in various trophoblastic cell lines. (A) The relative amount of the gene RITA in diverse trophoblastic cell lines. The results are presented as relative quantification (RQ) with minimum and maximum range, by setting the RITA value of BeWo cells as 1. (B) Western blot analysis with cellular extracts from HTR, BeWo, Jar, and JEG-3 using an antibody against RITA. β-actin served as loading control. (C) HTR and BeWo cells were transfected with GFP-RITA and stained for the microtubule marker acetylated α-tubulin and DNA (4’,6-diamidino-2-phenylindole-dihydrochloride (DAPI)). Representatives of the subcellular localization of RITA are shown. First and second row scale: 10 µm, third and fourth row scale: 5 µm.