Figure 6.
Dose-dependent reduction on HCV parameters upon treatment with a replication inhibitor. Huh-7 cells were inoculated with JFH-1 D183 virus at high multiplicity of infection (moi = 3) in the absence or presence of increasing amounts (0, 0.1, 0.33 and 1 μM) of the HCV polymerase inhibitor 2 mAd. 48 h later supernatants were collected, cell extracts were harvested and samples were subjected to the same analysis described in Figure 5. Note the proportional and dose response reduction of all viral parameters (RNA (A), infectivities (B,C) and protein (D)) in 2 mAd-treated cells and the absence of any significant effect in the assembly (E) and secretion (F) rates. The HCV RNA and the intracellular and extracellular infectivity levels in untreated cells were: 2 × 107 ± 2 × 106 HCV RNA copies per μg of total RNA, 2 × 104 ± 2.5 × 103 ffus per well, and 1.6 × 105 ± 2.5 × 104 ffus per ml of supernatant, respectively. Data shown are averages of two independent experiments, each one performed in duplicate (Mean; SD; n = 4). One-way ANOVA followed by Dunnett´s Multiple Comparison Test analysis was used for statistical significance analysis (* p < 0.05; ** p < 0.01; *** p < 0.001).