Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 2;8(12):1555. doi: 10.3390/cells8121555

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Erlin-1 protein down-regulation increases LD content in Huh-7 cells. siRNA-transfected Huh-7 cells were inoculated with JFH-1 D183 virus at high multiplicity of infection (moi = 3) and the intracellular LD content was analyzed 48 h after infection as described in Material and Methods. (A) Representative confocal image sections of intracellular LD accumulation (in green) in siRNA-transfected HCV-infected and uninfected cells. Nuclei (in blue) were counterstained with Hoechst dye. (B) Quantitation of the total LD fluorescence intensity signal per cell from confocal image sections. ImageJ software was used to quantitate the total LD fluorescence intensity signal in twenty individual cells of each condition. Each dot in the graph represents the LD fluorescence intensity of an individual cell and the horizontal lines show the average LD fluorescence intensity in each group of data. One-way ANOVA followed by Dunnett’s Multiple Comparison Test was used to determine the statistical significance (*** p < 0.001).