Figure 3.
A2AR-dependent cAMP accumulation. (A) Concentration-dependent effects of CGS21680 in cAMP accumulation in singly A2AR expressing cells. The signal was normalized by assigning the 100% to the maximum signal obtained and 0% to cells without ligand. The data are expressed as the mean ± SD of a representative experiment performed in triplicate. (B) Guanosine effects on CGS21680-mediated cAMP accumulation in cells expressing A2ARSNAP (blue bars) or A2ARSNAP plus A1RSNAP (red dashed bars). Results were normalized to the specific cAMP accumulation in the absence of guanosine for each transfection set and are expressed as mean ± SEM of four independent experiments performed in triplicate. The asterisks indicate statistically significant differences * p < 0.05, one-way ANOVA followed by Dunnett’s post-hoc testwhile compared to control (dashed line).