Figure 4.

Effect of treatment with the mast cell degranulation inhibitor or histamine receptor antagonists on vascular permeability induced by LiRecTCTP. Mice (n = 5) were pre-treated with promethazine (PRO), cimetidine (CIM), thioperamide (THIO), cromolyn (CROM), or PBS (control). Animals received solution of Evans blue dye in PBS intravenously prior to intradermal injection of LiRecTCTP (10 µg) or PBS (control). (A) Representative images of mice dorsal skin at the point of sample inoculation. (B) Quantitative measurement of dye leakage induced by LiRecTCTP and inhibition with CROM, PRO, CIM, and THIO. Data represent mean ± s.e.m of one representative experiment from three independent biological replicates (* p < 0.1 and ** p < 0.01).